Sensory and Motor Systems
Author: Marcela Sonia Vera | email: marceverita@gmail.com
Marcela Vera 1°2°, Sofía Stupniki 1°2°, Manuel Bruera 3°, María Constanza Paz 3°, Leonardo Dionisio 1°2°, Alan Vater 4°5°, Paula Schaiquevich 4°5°, María Ana Contín 3°, Guillermo Spitzmaul 1°2°
1° Instituto de Investigaciones Bioquímicas de Bahía Blanca (INIBIBB), CONICET/UNS.
2° Departamento de Biología, Bioquímica y Farmacia (BByF), UNS.
3° Departamento de Química Biológica de Córdoba, RC. CIQUIBIC-CONICET/UNC
4° Hospital de Pediatría J.P. Garrahan, Buenos Aires, Argentina
5° Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
Voltage-gated KCNQ potassium channel subunits are responsible for the M-current that regulates neuronal excitability. We found expression of KCNQ4 in the retinal pigmented epithelium (RPE) and in the ciliary body (CB) of mouse eyes, suggesting that it could participate in visual processing and aqueous humor formation. Using Kcnq4 knockout (KO) and wild-type (WT) mice we studied the role of KCNQ4 in vision. First, we analyzed Kcnq gene expression by qPCR. We found that in KO mice, the expression of Kcnq3 and -5 in the RPE/retina did not change. On the other hand, in CB the expression of Kcnq3 increased 2.5-fold while the expression of Kcnq5 decreased 30%. Then, we tested light perception by testing the innate aversion of rodents to it. We did not find any differences between the KO and WT in the test performance. To analyze the function of the neuronal visual pathway, we recorded electroretinogram (ERG) in both genotypes. We observed no differences in a- and b-wave peaks and latency times between WT and KO in young animals, whereas in 50 weeks-old mice we observed a reduction trend in b-wave peak in KO while a-wave showed no differences. We also measured intraocular pressure (IOP) in young animals to evaluate CB function. We found a slight increase in the IOP in KO mice (13.6±1.0 to 15.8±1.1 mm Hg). In conclusion, the presence of the KCNQ4 subunit is necessary for the proper expression of the other Kcnq subunits and it would contribute to CB and retinal function.